sgrna expression cassette Search Results


plasmid b270  (Addgene inc)
93
Addgene inc plasmid b270
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Plasmid B270, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid b270/product/Addgene inc
Average 93 stars, based on 1 article reviews
plasmid b270 - by Bioz Stars, 2026-03
93/100 stars
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dcas9-vp64 and u6-sgrna-cmv-mcherry expression cassettes  (Applied StemCell Inc)
90
Applied StemCell Inc dcas9-vp64 and u6-sgrna-cmv-mcherry expression cassettes
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Dcas9 Vp64 And U6 Sgrna Cmv Mcherry Expression Cassettes, supplied by Applied StemCell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dcas9-vp64 and u6-sgrna-cmv-mcherry expression cassettes/product/Applied StemCell Inc
Average 90 stars, based on 1 article reviews
dcas9-vp64 and u6-sgrna-cmv-mcherry expression cassettes - by Bioz Stars, 2026-03
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sgrna expression cassette sgrnapp3c14  (Twist Bioscience)
90
Twist Bioscience sgrna expression cassette sgrnapp3c14
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Sgrna Expression Cassette Sgrnapp3c14, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna expression cassette sgrnapp3c14/product/Twist Bioscience
Average 90 stars, based on 1 article reviews
sgrna expression cassette sgrnapp3c14 - by Bioz Stars, 2026-03
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sgrna expression cassette  (Azenta)
90
Azenta sgrna expression cassette
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Sgrna Expression Cassette, supplied by Azenta, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna expression cassette/product/Azenta
Average 90 stars, based on 1 article reviews
sgrna expression cassette - by Bioz Stars, 2026-03
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gblocks fragment containing 5 sgrna expression cassettes with high fidelity four-base overhang pair  (GenScript corporation)
90
GenScript corporation gblocks fragment containing 5 sgrna expression cassettes with high fidelity four-base overhang pair
a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 <t>gBlocks</t> with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and <t>eGFP</t> <t>sgRNA</t> plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
Gblocks Fragment Containing 5 Sgrna Expression Cassettes With High Fidelity Four Base Overhang Pair, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gblocks fragment containing 5 sgrna expression cassettes with high fidelity four-base overhang pair/product/GenScript corporation
Average 90 stars, based on 1 article reviews
gblocks fragment containing 5 sgrna expression cassettes with high fidelity four-base overhang pair - by Bioz Stars, 2026-03
90/100 stars
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sgrna expression cassettes  (Fasmac Co Ltd)
90
Fasmac Co Ltd sgrna expression cassettes
a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 <t>gBlocks</t> with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and <t>eGFP</t> <t>sgRNA</t> plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
Sgrna Expression Cassettes, supplied by Fasmac Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna expression cassettes/product/Fasmac Co Ltd
Average 90 stars, based on 1 article reviews
sgrna expression cassettes - by Bioz Stars, 2026-03
90/100 stars
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crrna-free sgrna expression cassette  (General Biosystems Inc)
90
General Biosystems Inc crrna-free sgrna expression cassette
a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 <t>gBlocks</t> with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and <t>eGFP</t> <t>sgRNA</t> plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
Crrna Free Sgrna Expression Cassette, supplied by General Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/crrna-free sgrna expression cassette/product/General Biosystems Inc
Average 90 stars, based on 1 article reviews
crrna-free sgrna expression cassette - by Bioz Stars, 2026-03
90/100 stars
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sgrna expression cassettes  (Eurofins)
90
Eurofins sgrna expression cassettes
a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 <t>gBlocks</t> with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and <t>eGFP</t> <t>sgRNA</t> plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
Sgrna Expression Cassettes, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna expression cassettes/product/Eurofins
Average 90 stars, based on 1 article reviews
sgrna expression cassettes - by Bioz Stars, 2026-03
90/100 stars
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sgrna expression cassettes  (Nature Biotechnology)
90
Nature Biotechnology sgrna expression cassettes
a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 <t>gBlocks</t> with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and <t>eGFP</t> <t>sgRNA</t> plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
Sgrna Expression Cassettes, supplied by Nature Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna expression cassettes/product/Nature Biotechnology
Average 90 stars, based on 1 article reviews
sgrna expression cassettes - by Bioz Stars, 2026-03
90/100 stars
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expression cassettes sgrna#5, sgrna#7, sgrna#21, sgrna#23, sgrna#24 and sgrna#25  (Twist Bioscience)
90
Twist Bioscience expression cassettes sgrna#5, sgrna#7, sgrna#21, sgrna#23, sgrna#24 and sgrna#25
a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 <t>gBlocks</t> with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and <t>eGFP</t> <t>sgRNA</t> plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
Expression Cassettes Sgrna#5, Sgrna#7, Sgrna#21, Sgrna#23, Sgrna#24 And Sgrna#25, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/expression cassettes sgrna#5, sgrna#7, sgrna#21, sgrna#23, sgrna#24 and sgrna#25/product/Twist Bioscience
Average 90 stars, based on 1 article reviews
expression cassettes sgrna#5, sgrna#7, sgrna#21, sgrna#23, sgrna#24 and sgrna#25 - by Bioz Stars, 2026-03
90/100 stars
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sgrna expression cassette  (Sangon Biotech)
90
Sangon Biotech sgrna expression cassette
a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 <t>gBlocks</t> with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and <t>eGFP</t> <t>sgRNA</t> plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
Sgrna Expression Cassette, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna expression cassette/product/Sangon Biotech
Average 90 stars, based on 1 article reviews
sgrna expression cassette - by Bioz Stars, 2026-03
90/100 stars
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sgrna expressing cassette sequences  (Bioneer Corporation)
90
Bioneer Corporation sgrna expressing cassette sequences
a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 <t>gBlocks</t> with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and <t>eGFP</t> <t>sgRNA</t> plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
Sgrna Expressing Cassette Sequences, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrna expressing cassette sequences/product/Bioneer Corporation
Average 90 stars, based on 1 article reviews
sgrna expressing cassette sequences - by Bioz Stars, 2026-03
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Image Search Results


KEY RESOURCES TABLE

Journal: Molecular cell

Article Title: CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons

doi: 10.1016/j.molcel.2017.08.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Plasmid: B270 (containing sgRNA targeting ATP1A1 + empty sgRNA-expressing cassette) , This paper , Available in Addgene.

Techniques: Virus, Subcloning, Recombinant, Transfection, DNA Extraction, PCR Cloning, Cloning, Plasmid Preparation, Software

a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 gBlocks with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and eGFP sgRNA plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.

Journal: bioRxiv

Article Title: Multiplex base editing to convert TAG into TAA codons in the human genome

doi: 10.1101/2021.07.13.452007

Figure Lengend Snippet: a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 gBlocks with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and eGFP sgRNA plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.

Article Snippet: All gBlocks fragment containing 5 sgRNA expression cassettes with high fidelity four-base overhang pair after cutting with type IIS restriction enzyme BbsI restriction enzyme were designed and directly sent to be synthesized into PUC57 cloning plasmid by GenScript.

Techniques: Modification, RNA Sequencing